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FeNO Test: Overview, Types, Causes & More
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FeNO Test
A FeNO test is one of the simplest ways doctors can detect if your airways are inflamed When your lungs are inflamed they might not
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A FeNO test is one of the simplest ways doctors can detect if your airways are inflamed. When your lungs are inflamed, they might not work as well as they ought to. FeNO stands for Fractional Exhaled Nitric Oxide, which is a particular gas that your body naturally makes and releases when your lungs are inflamed. In the decade of 1990s, the use of fractional exhaled nitric oxide (FeNO) started gaining momentum. During this time, researchers began recognizing that nitric oxide, a gas produced by cells in the body, was being exhaled by humans.

In the early 2000s, FeNO started becoming more popular. Today, many clinicians and researchers have realized the efficacy of the FeNO test in diagnosing asthma and other respiratory diseases. The significant reasons why FeNO testing became popular: 

  • It is a standardized testing
  • It is a non-invasive way to assess airway inflammation 
  • It can help doctors make better treatment decisions for people with respiratory conditions.

Researchers have examined how FeNO (fractional exhaled nitric oxide) can help in the evaluation of Asthma:

  • Managing Asthma in Pregnancy (MAP) trial: According to this study, kids who developed asthma around 4 to 6 years old had higher FeNO levels. Based on this study, many clinicians believe that FeNO can identify kids with a higher risk of getting asthma later.
  • Fractional exhaled Nitric Oxide (FeNO) in different asthma phenotypes study: This study stated that asthmatic people with allergies, like runny nose (allergic rhinitis) or itchy eyes (allergic conjunctivitis), had higher FeNO levels. 
  • Diagnostic accuracy of FeNO in asthma and predictive value for inhaled corticosteroid responsiveness study: According to this research, FeNO levels can predict whether individuals with asthma respond to inhaled corticosteroids. Thus, FeNO could help doctors decide the proper treatment for individuals with asthma.

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What are the types of Fractional Exhaled Nitric Oxide (FeNO) tests?

The Fractional Exhaled Nitric Oxide (FeNO) test itself doesn't have multiple types, but variations and considerations within the test can affect the results. The following are a few essential aspects to consider when discussing FeNO testing:

  • Phenotyping asthma: While distinct types of FeNO tests do not exist, FeNO levels can help characterize different asthma phenotypes. As a result, elevated FeNO levels often correlate with eosinophilic airway inflammation, a subtype of asthma marked by high levels of eosinophils in the airways. Thus, your doctor can create a treatment plan accordingly.

  • Standardization and interpretation: The doctor always follows a standardized approach to FeNO testing to have consistent and precise outcomes. Accurate interpretation of FeNO measurements involves factoring in patient-specific variables like age, sex, height, and other pertinent clinical details.

  • Treatment monitoring: Your doctor can use the FeNO test to monitor the effectiveness of anti-inflammatory medicines for managing respiratory conditions like asthma. The healthcare provider uses periodic FeNO measurements and can modify treatment plans based on observed trends.

  • Instrumentation variation: In FeNO measurement, several devices are available, each with specifications and operational protocols. The healthcare providers should adhere to the manufacturer's guidelines for achieving accurate results.

How does the FeNO test work?

How does the FeNO test work?

Non-invasive and minimally invasive, the FeNO test causes minor tissue damage. It measures the amount of nitric oxide (NO) we exhaled during breathing. NO is a gas produced by the cells lining the airways. It helps us in various functions, including:

  • Relaxing the airways 
  • Making breathing easier
  • Fighting infection
  • Reducing inflammation

In people with asthma, the airways are more inflamed than usual. This inflammation leads to an increase in NO production. As a result, people with asthma typically have higher NO levels in their exhaled breath than those without asthma.

The FeNO test helps in the following:

  • Diagnose asthma
  • Monitor the severity of asthma.
  • Assess the response to treatment.
  • Diagnose other conditions that can cause airway inflammation (chronic obstructive pulmonary disease (COPD) and allergic rhinitis).

The FeNO test is a relatively simple and quick assessment, and you can receive it in a doctor's office. It does not require any needles or incisions. The patient exhales into a device that measures the amount of NO in the breath. The results of the test are usually available within a few minutes.

  • The normal range for FeNO levels in children is below 20 parts per billion (ppb). Levels above 50 ppb are considered high and may indicate asthma.
  • The FeNO test can help diagnose asthma, to monitor the severity of asthma, and to assess the response to treatment. It helps diagnose other conditions like COPD and allergic rhinitis.

Why choose Medanta

Choosing Medanta for your FeNO (Fractional Exhaled Nitric Oxide) test is a decision rooted in careful consideration. As a doctor dedicated to your well-being, let me provide you with reasons that set Medanta apart:

  • Specialized expertise: At Medanta, you'll benefit from a team of accomplished medical professionals with extensive experience conducting and interpreting FeNO tests. 

  • Cutting-edge infrastructure: Medanta has advanced medical infrastructure, ensuring that your FeNO test uses advanced equipment for precision and accurate results.

  • Holistic approach: Our institution believes in providing comprehensive care. If your FeNO results indicate further steps, Medanta offers a range of specialties to ensure your overall health betterment.

  • Tailored treatment: With your FeNO test results, our specialized doctors can devise personalized treatment strategies, keeping your unique medical needs at the forefront.

  • Patient-centric philosophy: At Medanta, you are not just a patient but an integral part of your healthcare journey. We value your comfort preferences and actively involve you in care decisions.

  • Advancements in medicine: Being a prominent medical center, Medanta is often at the forefront of medical research and innovation, which means you benefit from the latest advances in healthcare.

  • Ease of access: Our facilities provide convenience and easy patient access. Your comfort in navigating your healthcare experience is a priority.

  • Reputation for excellence: As a leading provider of quality healthcare, Medanta is recognized by many people as a top choice among medical facilities.

  • Comprehensive services: The Comprehensive medical services offered by Medanta extend far beyond FeNO testing, giving you one place to go for all your healthcare needs.

Frequently Asked Questions about FeNO

What is FeNO?

FeNO stands for Fractional Exhaled Nitric Oxide, a measure of nitric oxide levels in a person's exhaled breath.

Why is the FeNO test essential for people who have asthma?

FeNO can be an essential diagnostic tool. It indicates airway inflammation and is particularly helpful for people who have asthma. 

How is FeNO measured?

FeNO is measured using a handheld device called a FeNO analyzer or monitor, which a person breathes into.

What are normal FeNO levels?

Normal FeNO levels can vary but are generally below 20 parts per billion (ppb) in children.

What does elevated FeNO indicate?

Elevated FeNO levels may suggest airway inflammation, indicating asthma, allergies, or other respiratory conditions.

Is FeNO testing invasive or uncomfortable?

FeNO testing is non-invasive and painless, involving simple breath collection.

With FeNO's help, can a doctor measure the efficacy of my symptoms?

Yes, with FeNO's help, the doctor can monitor the effectiveness of asthma treatment and create future treatment plans.

Is FeNO testing widely available?

FeNO testing is available in many healthcare facilities but may vary depending on location and resources.

Can FeNO help in diagnosing eosinophilic asthma?

FeNO helps in diagnosing eosinophilic asthma. Eosinophilic asthma is a subtype. An affected person develops high levels of eosinophils in the blood and inflammation in the airways.

Can FeNO testing help in the diagnosis of asthma in my child? 

The pediatric pulmonologist often employs FeNO testing in children to diagnose and manage asthma.

What conditions other than asthma can cause elevated FeNO levels?

Conditions like allergies, sinusitis, and viral infections can also cause elevated FeNO levels.

Does health insurance cover FeNO testing?

Health insurance may cover FeNO testing, but coverage policies vary, so you must check with your insurance provider.

Can FeNO levels change throughout the day?

FeNO levels can vary on various factors, including but not limited to: 

  • Time of day

  • Recent medication use

  • Exposure to allergens

How do healthcare providers interpret FeNO results?

When interpreting FeNO results, healthcare providers consider a person's age, medical history, and symptoms.

Does your tell the difference between allergic and non-allergic asthma with the help of the FeNO test?

FeNO testing can help your doctor identify whether you have allergic and non-allergic asthma.

How FeNO test can help me in managing my asthma?

With the help of FeNO testing, the doctor can create a personalized asthma treatment plan, thereby reducing your risk of over or under-medication.

Can FeNO levels be affected by diet or nutrition?

Diet and nutrition generally do not have a significant impact on FeNO levels.

Is FeNO testing recommended for all asthma patients?

FeNO testing may be recommended for asthma patients with uncontrolled symptoms or those who may benefit from personalized treatment adjustments.

How long does a FeNO test typically take to complete?

A FeNO test usually takes a few minutes, making it a quick and convenient diagnostic tool.

Can FeNO testing be done at home?

While FeNO testing is typically conducted in healthcare, portable devices with a prescription are available for at-home monitoring.

What are the safety considerations for FeNO testing?

FeNO is a safe test. FeNO is a non-invasive test. It causes no damage to tissues and is entirely safe. 

How does age affect normal FeNO levels?

Normal FeNO levels can vary with age, with higher levels seen in children and lower levels in adults.

Is FeNO testing suitable for individuals with other respiratory conditions besides asthma?

FeNO testing can help assess and manage respiratory conditions other than asthma that cause airway inflammation.

Are there any side effects of FeNO testing?

FeNO testing is generally free of side effects, as it involves simple exhalation into a device.

Can FeNO testing be used in conjunction with other asthma diagnostic tools?

FeNO testing is often used alongside other diagnostic methods, such as Forced Oscillometry Technique and allergy testing, to provide a comprehensive assessment.

How often should FeNO testing be repeated in asthma management?

The frequency of FeNO testing in asthma management depends on the individual's condition and treatment plan, typically ranging from a few weeks to several months.

What should a person expect during a FeNO test?

During a FeNO test, a person breathes into a mouthpiece or tube connected to a monitor while sitting comfortably.

Can FeNO testing help predict asthma exacerbations?

FeNO testing may provide insights that help predict asthma exacerbations, allowing for timely interventions.

Is FeNO testing suitable for people with severe asthma?

FeNO testing can help assess and manage severe asthma, helping refine treatment strategies.

Are there age restrictions for FeNO testing?

Children who are preschoolers and over are usually comfortable doing the test.

What role does FeNO play in personalized asthma treatment plans?

FeNO levels can guide the customization of asthma treatment plans, ensuring that medications are tailored to the individual's needs.

Can FeNO testing be performed during an asthma exacerbation?

Doctors can conduct FeNO testing during an asthma exacerbation, but results may be affected by the severity of symptoms.

What are the potential complications of untreated asthma indicated by high FeNO levels?

Untreated asthma with high FeNO levels can lead to ongoing airway inflammation, worsening symptoms, and reduced lung function.

Can FeNO levels help differentiate between allergic and non-allergic rhinitis?

FeNO levels may assist in distinguishing between allergic and non-allergic rhinitis, as allergic rhinitis often presents with elevated FeNO.

What role does FeNO play in asthma control and management plans for children?

FeNO testing is valuable in pediatric asthma management, aiding in assessing airway inflammation and treatment adjustments.

Can medication use influence FeNO levels?

Certain medications, such as inhaled corticosteroids, can lower FeNO levels by reducing airway inflammation.

How can patients prepare for a FeNO test?

Patients can prepare for a FeNO test by following any pre-test instructions provided by their healthcare provider, which may include avoiding certain foods or medications.

Can FeNO levels change with seasonal allergies?

FeNO levels may increase during allergy seasons when allergens trigger airway inflammation.


Can the FeNO test help in determining the effectiveness of allergen immunotherapy?

FeNO testing can help assess the effectiveness of allergen immunotherapy by monitoring changes in airway inflammation over time.

What factors can cause fluctuations in FeNO levels within an individual?

Fluctuations in FeNO levels within an individual can vary due to factors such as respiratory infections, exposure to irritants, and changes in treatment.

Is FeNO testing recommended for individuals with persistent cough and no other asthma symptoms?

FeNO testing may be considered in individuals with a persistent cough, as it can help identify airway inflammation without other asthma symptoms.

Can FeNO levels fluctuate due to stress or anxiety?

Stress or anxiety may lead to temporary fluctuations in FeNO levels, but these are generally insignificant for diagnostic purposes.


Can FeNO testing be affected by recent exercise or physical activity?

FeNO testing results may change due to recent intense exercise, so it's advisable to rest briefly before the test.

Can FeNO levels vary based on environmental factors like pollution?

Exposure to environmental factors like pollution may temporarily FeNO elevate levels.

Can FeNO testing help determine the need for asthma rescue medications?

FeNO testing may aid in determining the need for asthma rescue medications by assessing the degree of airway inflammation.

What is the role of FeNO in diagnosing exercise-induced bronchoconstriction (EIB)?

FeNO testing may assist in diagnosing exercise-induced bronchoconstriction (EIB) by assessing airway inflammation following exercise.

What is the role of FeNO in assessing treatment response in severe asthma cases?

FeNO testing can help assess treatment response in severe asthma cases, guiding adjustments to improve symptom control.

Can FeNO testing replace other asthma diagnostic methods like spirometry?

FeNO testing complements other asthma diagnostic methods like spirometry but does not replace them, as each provides unique information.

Can FeNO levels be affected by changes in humidity?

The values of FeNO levels can change with a change in humidity levels. Generally, high humidity can lead to lower FeNO measurements.

Can FeNO levels vary with age-related changes in lung function?

Age-related changes in lung function can impact FeNO levels, with older individuals typically having lower levels.

Can FeNO levels be affected by the presence of nasal polyps?

Nasal polyps can influence FeNO levels, and healthcare providers may consider this when interpreting results and assessing respiratory health.

Dr. Rajiv Uttam
Paediatric Care
Meet The Doctor
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